Presenters in this symposium focus on the varied functions of liver endothelial cells (ECs). ECs are specialized cells increasingly recognized for their heterogeneity and their key role in liver diseases and carcinogenesis. Recent advances in the understanding of EC function were made possible with refined isolation techniques coupled with multiomics studies. Speakers review emerging literature that defines liver ECs beyond their role as vascular barriers to critical mediators in various biological processes under physiologic and pathologic conditions.