Presence of receptors for polymerized albumin in HBsAg‐containing hepatocytes and hepatoma cell line

Swan N. Thung, Michael A. Gerber – 1 March 1981 – Recent evidence suggests that hepatitis B virions (HBV) and HBsAg particles contain receptors for polymerized human serum albumin (pHSA). We studied, by immunohistochemical techniques, the relationship between HBsAg and pHSA receptors in liver tissue from eight patients with chronic HBV infection and in a human hepatocellular carcinoma cell line (PLC/PRF/5) producing HBsAg. Both parallel sections and double fluorescent antibody staining of liver tissue demonstrated that only HBsAg‐containing hepatocytes expressed pHSA receptors.

Modification of galactosamine‐induced liver injury in rats by reticuloendothelial system stimulation or depression

Ali Al‐Tuwaijri, Kemal Akdamar, Nicholas R. di Luzio – 1 March 1981 – The reticuloendothelial system has been implicated in galactosamine‐induced liver injury because of a correlation between phagocytic alterations induced by colloidal carbon or endotoxin, and development of liver necrosis. To evaluate this concept, the influence of galactosamine on liver function and histology was determined in rats in which the reticuloendothelial system was normal, stimulated, or depressed.

Attenuation of the ethanol‐induced hepatic redox change after chronic alcohol consumption in baboons: Metabolic consequences in vivo and in vitro

Mikko P. Salaspuro, Spencer Shaw, Elizabeth Jayatilleke, William A. Ross, Charles S. Lieber – 1 January 1981 – Acute ethanol administration results in increased hepatic NADH/NAD+ ratio and inhibition of galactose elimination, tricarboxylic acid cycle activity, and fatty acid oxidation. To determine how this redox change is affected by chronic alcohol consumption and to assess the resulting metabolic consequences, we studied baboons which were fed alcohol as 50% of their total calories.

Pathology of cytoskeleton of liver cells: Demonstration of mallory bodies (alcoholic hyalin) in murine and human hepatocytes by immunofluorescence microscopy using antibodies to cytokeratin polypeptides from hepatocytes

Helmut Denk, Werner W. Franke, Brigitte Dragosics, Ingrid Zeiler – 1 January 1981 – A cytoskeleton fraction enriched in intermediate‐sized filaments and resistant to high and low salt buffer containing Triton X‐100 was prepared from dissociated mouse liver cells and mouse liver homogenates. One‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis revealed a family of polypeptides in the relative molecular weight (Mr) range from 41,000 to 55,000, with two polypeptides (component A, Mr 55,000; component D, Mr 48,000) as major constituents.

Patterns of hypothalamic‐pituitary‐gonadal dysfunction in men with liver disease due to differing etiologies

David H. van Thiel, Judith S. Gavaler, Joel A. Spero, Kimberley M. Egler, Carl Wight, Ajitkumar T. Sanghvi, Ute Hasiba, Jessica H. Lewis – 1 January 1981 – The hypothalamic‐pituitary‐gonadal axis was evaluated in two groups of age‐matched men with documented biochemical and histologic liver disease and compared to that of age‐matched normal controls.

Hepatitis B viral markers in severe viral hepatitis: Influence of steroid therapy

Harry B. Greenberg, William S. Robinson, C. Michael Knauer, Peter B. Gregory – 1 January 1981 – In our double‐blind randomized trial of methylprednisolone vs. placebo in severe viral hepatitis, 16 patients with hepatitis B (8 on steroid, 8 on placebo) were followed for at least 4 weeks. Four of the eight patients receiving methylprednisolone eventually died and all patients on placebo survived. Despite marked reduction in serum IgG in steroid‐treated patients, the decline in HBsAg titer and disappearance of Dane particle markers was the same in both treatment groups.

Subscribe to