Giuliana Montosi, Cinzia Garuti, Sabrina Martinelli, Antonello Pietrangelo – 30 December 2003 – Oxidant stress plays a key role in hepatic fibrogenesis. This study was undertaken to assess whether, during iron overload–associated liver fibrosis ain vivo, oxidant stress occurs in hepatic stellate cells (HSC) during active fibrogenesis. Gerbils were treated with iron‐dextran, and, after hepatic fibrosis developed, livers were subjected to various combination of in situ hybridization and immunocytochemistry analyses.

Thierry Poynard, Pierre Opolon – 30 December 2003

Jens‐Gerd Scharf, Thomas Knittel, Frank Dombrowski, Lars Müller, Bernhard Saile, Thomas Braulke, Heinz Hartmann, Giuliano Ramadori – 30 December 2003 – The insulin‐like growth factors I and II (IGF‐I, ‐II) are circulating peptides known to participate in the regulation of metabolism, growth, and cellular differentiation. In the present study, “early cultured” (days 2‐3 of culture) and “culture‐activated” (days 6‐7 of culture) rat hepatic stellate cells (HSCs) were analyzed for expression of individual components of the IGF axis.

Norifumi Kawada, Shuichi Seki, Masayasu Inoue, Tetsuo Kuroki – 30 December 2003 – Effects of antioxidants, resveratrol, quercetin, and N‐acetylcysteine (NAC) on the functions of cultured rat hepatic stellate cells and Kupffer cells were studied. These compounds dose‐dependently suppressed serum‐dependent proliferation of stellate cells as determined by [3H]thymidine and 5‐bromo‐2′‐deoxyuridine uptake. Expression of smooth muscle α‐actin was suppressed by a high dose of resveratrol and quercetin.

Miguel Navasa, Antonio Follo, Xavier Filella, Wladimiro Jiménez, Anna Francitorra, Ramón Planas, Antoni Rimola, Vicente Arroyo, Joan Rodés – 30 December 2003 – Spontaneous bacterial peritonitis (SBP) is associated with an important production of inflammatory mediators. However, it is unknown whether there is a relationship between the abdominal production of these mediators and the development of renal impairment, one of the most important prognostic parameters in spontaneous bacterial peritonitis.

Henning Andersen, Mette Borre, Johannes Jakobsen, Per Heden Andersen, Hendrik Vilstrup – 30 December 2003 – To study motor function quantitatively in alcoholic liver cirrhosis muscle strength, liver function, peripheral nerve function, and nutrition were assessed in 24 patients. Isokinetic strength of flexion and extension at elbow, wrist, hip, knee, and ankle and of shoulder abduction and adduction was evaluated and compared with findings in 24 matched healthy subjects. Degree of liver disease was assessed with the Child‐Pugh score and the galactose elimination capacity (GEC).

Marta Bosch‐Marcé, Manuel Morales‐Ruiz, Wladimiro Jiménez, Nuria Bordas, Manuel Solé, Josefa Ros, Ramón Deulofeu, Vicente Arroyo, Francisca Rivera, Juan Rodés – 30 December 2003 – This article assesses the circulating levels of l‐arginine, the renal response to l‐arginine infusion, and the renal expression of inducible and constitutive nitric oxide synthase (NOS II and NOS III, respectively) in cirrhotic rats with ascites. Systemic and renal hemodynamics and renal function were measured in basal conditions and following two doses of l‐arginine (5 and 10 mg · kg−1 · min for 40 minutes).

30 December 2003

Barbara Scheiber, Hans Goldenberg – 30 December 2003 – The chelator diethylenetriaminepentaacetate (DTPA) forms a stable complex with iron that does not donate iron to transferrin under physiological conditions, i.e., pH above 7 and isotonic milieu. It does, however, deliver iron to hepatocytes. This uptake is initiated by a mobilization of the metal from the complex by the cell surface. When an external chelator is added simultaneously, it can bind the iron and inhibit its accumulation by the cells.

Sylke Roth, Kristin Michel, Axel M. Gressner – 30 December 2003 – Cultured parenchymal liver cells (PC) were recently recognized to contain (latent) transforming growth factor β (TGF‐β) while the expression of TGF‐β mRNA remains controversial. This study was designed to analyze PC in different microenvironments (liver in situ, highly purified, isolated, and cultured PC) regarding the qualitative and quantitative content of mature and latent TGF‐β protein (immunostainings, enzyme‐linked immunosorbent assay [ELISA], and enzyme‐labeled fluorescence [ELF] technique).