Man‐Fung Yuen, Erwin Sablon, Chee‐Kin Hui, He‐Jun Yuan, Hilde Decraemer, Ching‐Lung Lai – 30 December 2003 – Factors associated with hepatitis B virus (HBV) DNA breakthrough and the significance of YMDD variants without the presence of wild‐type YMDD during prolonged lamivudine treatment are unknown. We studied the amino acid sequence of codon 552 (YMDD motif) and codon 528 by means of a line probe assay in 159 chronic HBV patients (median follow‐up 29.6 months).

Jonathan Larkin, Marcia M. Clayton, Jie Liu, Mark A. Feitelson – 30 December 2003 – Epidemiologic observations show a higher frequency of hepatitis B virus (HBV) serologic markers in chronic alcoholics compared with the general population. This may be the result of an increased susceptibility of alcoholics to infection and/or to an ethanol‐mediated stimulation of HBV gene expression and replication. To test the latter hypothesis, HBV transgenic SCID mice, which support consistent levels of virus replication, were fed with a standard Lieber‐DiCarli or isocaloric diet for 5 weeks.

Wilhelm Schneiderhan, Alexandra Schmid‐Kotsas, Jinshun Zhao, Adolf Grünert, Andreas Nüssler, Hans Weidenbach, Andre Menke, Roland M. Schmid, Guido Adler, Max G. Bachem – 30 December 2003 – Cumulating evidence suggests that oxidative stress resulting in lipid peroxidation and protein modification is involved in the pathogenesis of chronic liver injury and fibrogenesis. We investigated the effects of oxidized low‐density lipoproteins (oxLDL) on collagen and fibronectin synthesis of cultured human and rat hepatic stellate cells (HSC).

Valerie Paradis, Gabriel Perlemuter, Franck Bonvoust, Delphine Dargere, Beatrice Parfait, Michel Vidaud, Marc Conti, Stephane Huet, Nathalie Ba, Catherine Buffet, Pierre Bedossa – 30 December 2003 – Nonalcoholic steatohepatitis (NASH) may progress to liver fibrosis and cirrhosis. Mechanisms directly involved in the development of fibrosis have been poorly investigated.

Wei‐Chen Lee, Hui‐Chuan Wang, Long‐Bin Jeng, Yang‐Jen Chiang, Chen‐Rong Lia, Pei‐Fang Huang, Miin‐Fu Chen, Shiguang Qian, Lina Lu – 30 December 2003 – Hepatocellular carcinoma (HCC) is a common malignancy with a poor prognosis. This investigation examined whether dendritic cell–based immunotherapy can treat murine HCC effectively. Bone marrow–derived dendritic cells were propagated from C57BL/10J mice in GM‐CSF (4 ng/mL) and interleukin (IL)‐4 (1,000 μ/mL). The dendritic cells were pulsed with a Hepa1‐6 lysate overnight and employed to treat murine HCC.

Miwako Fujioka, Yutaka Nakashima, Osamu Nakashima, Masamichi Kojiro – 30 December 2003 – Sixty‐eight cases of single hepatocellular carcinoma (HCC) with less than 3 cm of diameter were immunohistochemically examined for the expressions of α‐fetoprotein (AFP) and protein induced by vitamin K absence or antagonist II (PIVKA‐II). In cancerous tissues, the expression rate was significantly higher for PIVKA‐II (34 cases [50%]) than AFP (21 cases [31%]) (P < .05), suggesting a higher specificity of PIVKA‐II to small HCC.

D. Montgomery Bissell – 30 December 2003

Rainer Gosert, Darius Moradpour – 30 December 2003

Javier Fernández, Miquel Navasa, Juliá Gómez, Jordi Colmenero, Jordi Vila, Vicente Arroyo, Juan Rodés – 30 December 2003 – The extensive use of invasive procedures and of long‐term norfloxacin prophylaxis in the management of cirrhotic patients may have influenced the epidemiology of bacterial infections in cirrhosis. We conducted a prospective evaluation of all bacterial infections diagnosed in patients with cirrhosis in a Liver Unit between April 1998 and April 2000. A total of 405 patients presented 572 bacterial infections in 507 admissions.

Elena Obrador, Julian Carretero, Angel Ortega, Ignacio Medina, Vicente Rodilla, José A. Pellicer, José M. Estrela – 30 December 2003 – B16 melanoma (B16M) cells with high glutathione (GSH) content show rapid proliferation in vitro and high metastatic activity in the liver in vivo. γ‐Glutamyl transpeptidase (GGT)‐mediated extracellular GSH cleavage and intracellular GSH synthesis were studied in vitro in B16M cells with high (F10) and low (F1) metastatic potential. GGT activity was modified by transfection with the human GGT gene (B16MF1/Tet‐GGT cells) or by acivicin‐induced inhibition.