Vincent Agnello, György Ábel, Glenn B. Knight, Elizabeth Muchmore – 30 December 2003 – The controversial question of the extent of hepatocyte infection in chronic hepatitis C was re‐examined in both chimpanzees and humans using a newly modified in situ hybridization (ISH) method for detecting hepatitis C virus (HCV) RNA. The specificity of the methodology for distinguishing positive‐ and negative‐strand synthetic HCV RNA was at least six magnitudes greater than the reverse‐transcription polymerase chain reaction (RT‐PCR) assay for HCV.

W. Michael McDonnell – 30 December 2003

30 December 2003

Jiezhong Chen, Graham Robertson, Jacqueline Field, Christopher Liddle, Geoffrey C. Farrell – 30 December 2003 – Gender differences in hepatic sex steroid and drug metabolism result from hormonal regulation of specific cytochrome P450 genes (CYP). In male rats, bile duct ligation (BDL) is associated with down‐regulation of the male‐specific genes, CYP2C11 and CYP3A2, together with a decrease in serum testosterone levels and a two‐ to threefold increase in serum estradiol concentrations.

The Cancer of the Liver Italian Program (Clip) Investigators – 30 December 2003 – The clinical outcome of cirrhotic patients with hepatocellular carcinoma (HCC) depends both on the residual liver function and tumor characteristics. However, the relative prognostic weight of these variables is not well defined. The aims of this study were to verify the value of known prognostic factors and to devise a prognostic index more sensitive than the commonly used Okuda stage. A retrospective analysis of the cases of HCC diagnosed at 16 Italian institutions from 1990 to 1992 was performed.

Thelma E. Wiley, Mary McCarthy, Lama Breidi, Monica McCarthy, Thomas J. Layden – 30 December 2003 – In patients infected with the hepatitis C virus (HCV), 20% to 30% will progress to cirrhosis in over two to three decades. Viral and host factors that are important in the clinical and histologic progression of HCV infection are not entirely certain. It has been suggested that liver disease is worse in alcoholics infected with HCV.

Pere Ginés, Tomas Berl, Mauro Bernardi, Daniel G. Bichet, Guilles Hamon, Wladimiro Jiménez, Jean‐François Liard, Pierre‐Yves Martin, Robert W. Schrier – 30 December 2003

Jacquelyn J. Maher – 30 December 2003

Goro Sato, Shoichi Matsutani, Hitoshi Maruyama, Hiromitsu Saisho, Takeshi Fukuzawa, Hideaki Mizumoto, Naoto Burioka – 30 December 2003

Pilar Bravo, Virginie Bender, Doris Cassio – 30 December 2003 – Efficient transport of bile acids, a typical characteristic of hepatocytes, is partially lost in most hepatoma cell lines and in normal hepatocytes after some days in culture. We have tested whether the polarized rat hepatoma–human fibroblast hybrid WIF (hybrids between W138 and Fao cells) cells previously obtained by our group were able to perform vectorial transport of the fluorescent bile acid derivative cholylglycylamidofluorescein (CGamF) towards the bile canaliculi (BC). Four different WIF clones were analyzed.