Reduced glutathione depletion causes necrosis and sensitization to tumor necrosis factor‐α–induced apoptosis in cultured mouse hepatocytes

Hidenari Nagai, Katsuhiko Matsumaru, Guoping Feng, Neil Kaplowitz – 30 December 2003 – The effect of reduced glutathione (GSH) depletion by acetaminophen (APAP), diethylmaleate (DEM), or phorone on the mode of cell death and susceptibility to tumor necrosis factor (TNF)‐induced cell death was studied in cultured mouse hepatocytes. Dose‐dependent necrosis was the exclusive mode of cell death with APAP alone, but the addition of TNF‐α induced a switch to about half apoptosis without changing total loss of viability.

Differential regulation of cyclins D1 and D3 in hepatocyte proliferation

David G. Rickheim, Christopher J. Nelsen, John T. Fassett, Nikolai A. Timchenko, Linda K. Hansen, Jeffrey H. Albrecht – 30 December 2003 – Substantial evidence suggests that cyclin D1 plays a pivotal role in the control of the hepatocyte cell cycle in response to mitogenic stimuli, whereas the closely related protein cyclin D3 has not been extensively evaluated. In the current study, we examined the regulation of cyclins D1 and D3 during hepatocyte proliferation in vivo after 70% partial hepatectomy (PH) and in culture.

Transferrin receptor gene expression and transferrin‐bound iron uptake are increased during postischemic rat liver reperfusion

Lorenza Tacchini, Daniela Fusar Poli, Aldo Bernelli‐Zazzera, Gaetano Cairo – 30 December 2003 – Iron‐catalyzed production of reactive oxygen species is a cause of liver injury after ischemia/reperfusion (I/R). The aim of the present study was to address the regulation of transferrin receptor (TfR), which mediates cellular iron uptake, during I/R. The molecular mechanisms controlling TfR gene expression in vivo during I/R of rat liver were investigated by molecular biology procedures. We also analyzed transferrin‐bound iron uptake into surviving liver slices.

Interactions of rifamycin SV and rifampicin with organic anion uptake systems of human liver

Stephan R. Vavricka, Jessica Van Montfoort, Huy Riem Ha, Peter J. Meier, Karin Fattinger – 30 December 2003 – The antibiotics rifamycin SV and rifampicin substantially reduce sulfobromophthalein (BSP) elimination in humans. In rats, rifamycin SV and rifampicin were shown to interfere with hepatic organic anion uptake by inhibition of the organic anion transporting polypeptides Oatp1 and Oatp2. Therefore, we investigated the effects of rifamycin SV and rifampicin on the OATPs of human liver and determined whether rifampicin is a substrate of 1 or several of these carriers.

Effects of noradrenalin and albumin in patients with type I hepatorenal syndrome: A pilot study

Christophe Duvoux, David Zanditenas, Christophe Hézode, Anthony Chauvat, Jean‐Luc Monin, Françoise Roudot‐Thoraval, Ariane Mallat, Daniel Dhumeaux – 30 December 2003 – Treatment of hepatorenal syndromes (HRSs) is currently based on vasopressin analogs. The aim of this pilot study was to evaluate the efficacy and safety of noradrenalin (NA) in the treatment of type 1 HRS.

Cell adhesion regulates platelet‐derived growth factor–induced MAP kinase and PI‐3 kinase activation in stellate cells

Vinicio Carloni, Raffaella M. S. DeFranco, Alessandra Caligiuri, Alessandra Gentilini, Silvia Cappadona Sciammetta, Elisabetta Baldi, Benedetta Lottini, Paolo Gentilini, Massimo Pinzani – 30 December 2003 – The biologic effects of growth factors are dependent on cell adhesion, and a cross talk occurs between growth factors and adhesion complexes. The aim of the present study was to evaluate the influence of cell adhesion on the major intracellular signaling pathways elicited by platelet‐derived growth factor (PDGF) in hepatic stellate cells (HSC).

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