Reverse transcriptase‐polymerase chain reaction fails to detect peripheral‐blood hepatitis C RNA in formalin‐fixed liver tissue
Rubén Bonilla Guerrero, Kenneth P. Batts, Jeffrey J. Germer, Rogelio G. Perez, Russell H. Wiesner, David H. Persing – 30 December 2003 – Currently, one of the major indications for liver transplantation is infection with hepatitis C virus (HCV). Many studies have suggested that recurrent infection with HCV is universal after transplantation. Fastidious techniques, such as reverse transcriptase‐polymerase chain reaction (RT‐PCR), have proved to be highly sensitive for detecting HCV RNA in serum and in fresh‐frozen and formalin‐fixed paraffin‐embedded (FFPE) liver tissue.