T Miyamura – 1 August 1996

1 August 1996

E Windler, J Greeve, H Robenek, F Rinninger, H Greten, S Jäckle – 1 August 1996 – Initial binding and subsequent endocytosis of small and large chylomicron remnants by rat liver were compared. Small and large chylomicrons were obtained from mesenteric lymph of glucose‐ or fat‐fed rats, respectively. The low‐density lipoprotein (LDL) receptor was up‐ and down‐regulated as shown by LDL receptor messenger RNA (mRNA). The rate of removal of small chylomicron remnants by isolated perfused rat livers followed closely the activity of the LDL receptor.

T Knittel, T Janneck, L Muller, P Fellmer, G Ramadori – 1 August 1996 – During liver fibrogenesis, Ito cells are regarded as the principal matrix synthesizing cells and transforming growth factor β 1 (TGF‐β 1) appears to be the main fibrogenic mediator. This study analyzed the effects of TGF‐β 1 on Ito cell activation, proliferation, and on the expression of a set of matrix proteins, antiproteases, and TGF‐β receptors both in “early cultured” and “culture‐activated” Ito cells.

M A Dingemanse, W J De Jonge, P A J De Boer, M Mori, W H Lamers, A F Moorman – 1 August 1996 – Ammonia‐fixation in mammalian livers is, via the ornithine cycle and glutamine synthetase, strictly compartmentalized, occurring in a wide upstream periportal compartment and in the very last downstream pericentral hepatocytes, respectively. This conclusion is based on the well‐known distribution patterns of carbamoyl phosphate synthetase I (CPS) and glutamine synthetase in the developing and adult liver.

J Chen, W Song, R N Redinger – 1 August 1996 – The effect of 2‐week 2% cholesterol vs. chow feeding on regulation of hepatic lipoprotein, lipids and apoprotein (Apo), and biliary lipids production was evaluated by the isolated perfused hamster liver model. Cholesterol feeding did not change very‐low‐density lipoprotein (VLDL), low‐density lipoprotein (LDL), and high‐density lipoprotein (HDL) particle size but significantly increased the hepatic production of VLDL‐cholesterol fourfold, VLDL‐triglyceride two and one‐half‐fold but not phospholipid in VLDL.

P E Ballmer, J Reichen, M A McNurlan, A B Sterchi, S E Anderson, P J Garlick – 1 July 1996 – Albumin and fibrinogen synthesis rates were measured in 15 subjects with different clinical stages of postviral cirrhosis and compared with galactose elimination capacity and aminopyrin breath test. Forty‐three mg per kg body weight [2H5ring]phenylalanine with an isotopic enrichment of 10 atom% were intravenously injected. [2H5ring]phenylalanine enrichments in the plasma‐free phenylalanine and the albumin and fibrinogen isolates were measured by gas chromatography‐mass spectrometry.

S M Jones, R G Thurman – 1 July 1996 – A low‐flow, reflow model of liver perfusion was used in the rat to investigate the effects of L‐arginine on reperfusion injury in the absence of blood elements. In contrast to in vivo studies, L‐arginine cannot minimize hypoxia by improving the microcirculation under these special conditions, but rather can only increase oxygen delivery upon reflow. During reflow, lactate dehydrogenase (LDH) release reached a new steady‐state value of 35 ± 3 U/g/h in livers perfused in the absence of L‐arginine.

E C Torchia, R J Shapiro, L B Agellon – 1 July 1996 – The liver recovers bile acids from the portal circulation primarily via an active process that is dependent on sodium ions. Hepatocytes lose the ability to transport bile acids in culture, and, in liver‐derived permanent cell lines, this ability is severely reduced or absent. To study the importance of bile acids in regulating liver‐specific functions (e.g., cellular bile acid and cholesterol metabolism), we have re‐established active bile acid transport in cultured cells.

M Mohler, W Stremmel – 1 July 1996