Heat serum inactivation as a mandatory procedure for antiactin antibody detection in cell culture
E L Cancado, L S Vilas‐Boas, C P Abrantes‐Lemos, N F Novo, G Porta, L C Da Silva, A A Laudanna – 1 May 1996 – In autoimmune hepatitis (AIH), the smooth‐muscle antibody is specific for polymerized actin. Detection of antiactin antibody (AAA) has been hampered by technical problems. We have investigated AAA in 30 sera from patients with liver diseases and smooth‐muscle antibody. AAA was detected by indirect immunofluorescence in 1:40, 1:80, and 1:160 dilutions.