A E Sirica – 30 December 2003

F von Weizsäcker, S Wieland, J Köck, W Offensperger, S Offensperger, D Moradpour, H E Blum – 30 December 2003

J W Marsh, I Dvorchik, M Subotin, V Balan, J Rakela, E P Popechitelev, V Subbotin, A Casavilla, B I Carr, J J Fung, S Iwatsuki – 30 December 2003 – Orthotopic liver transplantation (OLTx) in the presence of hepatocellular carcinoma (HCC) has been complicated by high recurrence rates. The ability to determine the risk and timing of HCC recurrence on an individual basis would greatly aid in the candidate selection process resulting in a more efficient use of donated organs and allow the individualization and better evaluation of adjuvant chemotherapy.

M Forestier, M Solioz, F Isbeki, C Talos, J Reichen, S Krahenbuhl – 30 December 2003 – It is well known that the hepatic mitochondrial protein content is increased in rats 4 weeks after bile duct ligation. In the present study, we measured the time course of this increase and assessed the levels of selected mitochondrial messenger RNA (mRNA) species and the rate of mitochondrial protein synthesis by isolated mitochondria.

Y Kitamura, T Tanigawa, T Katsumoto, K Tomita, H Wang, K Hirai, K Ichihara, T Terada – 30 December 2003 – A novel mouse Ito (fat‐storing) cell line (A640‐IS) was established by transformation with a temperature‐sensitive mutant of simian virus 40 (SV40) and the relationships between the expression of SV40 large T antigen and the growth, differentiation, and functions of A640‐IS cells were investigated. A640‐IS cells expressed large T antigen when cultured at 33 degrees C. At this temperature, the cells grew actively, assumed a fibroblastic shape, and showed few Ito cell characteristics.

A Preaux, A Mallat, J Rosenbaum, E Zafrani, P Mavier – 30 December 2003 – During the course of liver fibrogenesis, myofibroblast‐like cells (MFLC), mostly derived from hepatic stellate cells, proliferate and synthesize excessive amounts of extracellular matrix components. Pentoxifylline (PTX) elicits antiproliferative and antifibrogenic effects in human dermal fibroblasts. The aim of this study was to test the effects of PTX on the proliferation and the synthesis of collagen and gelatinase A in cultured human hepatic MFLC. MFLC were obtained by outgrowth from human liver explants.

C Bernadich, J C Bandi, C Piera, J Bosch, J Rodes – 30 December 2003 – Total portal‐systemic shunting in normal animals is associated with splanchnic and systemic vasodilation, suggesting that vasodilation in cirrhosis may be facilitated by spontaneous shunts promoted by portal hypertension. However, the mechanism of this vasodilation is unknown. The aim is to study the acute effects of the graded diversion of portal blood flow to the systemic circulation in normal and portal hypertensive rats.

H Imamura, B Rocheleau, J Côté, P M Huet – 30 December 2003 – Our aim was to investigate the time‐related changes in various parameters following orthotopic rat liver transplantation with (AOLT) and without (NOLT) arterial reconstruction in male Lewis rats. Body weight and biochemical parameters were measured weekly, and a liver biopsy was obtained at 4, 8, and 12 weeks. Hemodynamics were evaluated at 12 weeks using the microsphere technique and compared with matched controls. Following AOLT, rats gained weight normally without any noticeable complication.

A M Madden, W Bradbury, M Y Morgan – 30 December 2003 – Impairment of gustatory acuity may influence nutrient intake and hence nutritional status. The aim of this study was to evaluate gustatory acuity in patients with cirrhosis and its relationship to circulating concentrations of micronutrients, and food preferences. Gustatory evaluation was undertaken, using a rinsing technique, in 75 cirrhotic patients and 75 comparable healthy volunteers. Circulating concentrations of magnesium, zinc, vitamin A, and α‐ and β‐carotene were measured, and food preferences were assessed by questionnaire.

C Merkel, D Sacerdoti, M Bolognesi, E Enzo, R Marin, G Bombonato, P Angeli, A Gatta – 30 December 2003 – The association β‐blockers plus isosorbide‐5‐mononitrate (I5M) has been proposed for the treatment of portal hypertension in patients with insufficient response to β‐blockers alone, according to hemodynamic criteria. The mechanism of action in these patients is not clearly defined.