Cytokine gene expression by Kupffer cells in experimental alcoholic liver disease

Seiichiro Kamimura, Hidekazu Tsukamoto – 1 October 1995 – Kupffer cell‐derived cytokines are believed to play pivotal paracrine roles in the pathogenesis of alcoholic liver disease (ALD). To evaluate this hypothesis, Kupffer cell gene expression of tumor necrosis factor‐alpha (TNFα), interleukin (IL)‐6, and transforming growth factor‐beta 1 (TGFβ1) were directly examined in the rat model of ALD. Kupffer cells were isolated from the model after 10 and 17 weeks of intragastric ethanol infusion. These two durations resulted in focal hepatocellular injury and liver fibrogenesis, respectively.

Differential expression of A and B laminin chains during rat liver regeneration

América Giménez, Joaquim Hostench, Stamatis C. Stamatoglou, Carlos Enrich – 1 October 1995 – We have studied the distribution and expression of laminin during rat liver regeneration by immunofluorescence and immunoblotting using affinity‐purified laminin antibodies. Laminin was localized on sinusoidal surfaces in normal and regenerating hepatic parenchyma, but enhanced expression was detected during regeneration from 6 hours to 7 days after a partial hepatectomy.

Effects of liver transplantation on bile formation and biliary lipid secretion in the Sprague‐Dawley rat

Francis K. L. Chan, Yikun Zhang, Francis R. Sutherland, Eldon A. Shaffer – 1 October 1995 – Altered hepatic secretory function after orthotopic liver transplantation constitutes a major perioperative clinical problem. Cholestasis and cholesterol gallstone formation are among the most frequent complications reported. Such changes in the allograft secretory function can be secondary to many factors like graft injury due to preservation and marked rejection, surgical complications, immunosuppressive therapy, and sepsis.

The liver‐spleen scan as a quantitative liver function test: Correlation with liver severity at peritoneoscopy

John C. Hoefs, Felix Wang, Gary Kanel, Philip Braunstein – 1 October 1995 – Sulfur colloid distribution on liver‐spleen scan is determined by the perfused Kupffer cell mass. The perfused Kupffer cell mass is proportional to the perfused hepatocyte mass, but is less affected by acute changes in hepatocyte function. Thus, sulfur colloid distribution parameters (precisely measured by quantitative liver‐spleen scan [QLSS]) may be an excellent test of the perfused hepatic mass.

Inactivation of kupffer cells after prolonged donor fasting improves viability of transplanted hepatic allografts

Howard N. Sankary, Anita Chong, Preston Foster, Esther Brown, Jikun Shen, Robert Kimura, Guarimella Rayudu, James Williams – 1 October 1995 – Data from recent studies suggest that donor fasting imparts a beneficial effect on the viability of transplanted hepatic allografts. Because starvation may temporarily inactivate Kupffer cells, and because these cells are the likely mediators of liver injury after prolonged preservation‐reperfusion, the purpose of this study is to establish a link between improved organ viability and Kupffer cell inactivation caused by donor allograft fasting.

Anti‐hepatitis B virus activity of a mixture of two monoclonal antibodies in an “inhibition in solution” assay

Rudolf A. Heijtink, Johannes Kruining, Yvonne A. M. Weber, Robert A. de Man, Solko W. Schalm – 1 October 1995 – Two human monoclonal antibodies with anti‐hepatitis B activity were investigated separately and as a mixture by means of an “inhibition in solution” assay. With this assay the capacity of anti‐HBs antibodies to inhibit the binding of hepatitis B surface antigen (HBsAg) with solid‐phase anti‐HBs (Ausria II, Abbott Laboratories) was studied. Both HBsAg of different subtypes and purified Dane particles were used.

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