Mark E. Peeples – 1 November 1994 – In a previous study, we have identified endonexin II (E‐II) on human liver plasma membranes as a specific, Ca‐dependent, small hepatitis B surface antigen (HBsAg)‐binding protein. In this article, we describe the spontaneous development of anti‐HBs antibodies in rabbits immunized with native or recombinant human liver E‐II and in chickens immunized with the F(ab′)2 fragment of rabbit anti‐human liver E‐II immunoglobulin G. Anti‐HBs activity was not observed in rabbits immunized with rat liver E‐II.

Iljia Toshkov, Francis V. Chisari, Peter Bannasch – 1 November 1994 – Hepatocarcinogenesis in hepatitis B virus transgenic mice was studied by means of a correlative cytomorphological and cytochemical approach at different time points in animals from 1 to 34 mo old. HBsAg‐positive ground‐glass hepatocytes emerged throughout the liver parenchyma in nearly all transgenic mice during the first 4 mo after birth. The panlobular expression of HBsAg persisted until foci of altered hepatocytes appeared (6 to 9 mo of age).

Akihiro Tamori, Shuhel Nishiguchi, Tetsuo Kuroki, Shuichi Seki, Kenzo Kobayashi, Hiroaki Kinoshita, Shuzo Otani – 1 November 1994 – To evaluate the correlation between the polyamine metabolism and the degree of malignancy in hepatocellular carcinoma, we measured ornithine decarboxylase activity and polyamine concentrations in neoplastic tissue and adjacent noncancerous tissue from resected specimens of liver from 30 patients.

Paolo Caraceni, Antonio Gasbarrini, Andreas Nussler, Mauricio Di Silvio, Fabio Bartoli, Andre B. Borle, David H. Van Thiel – 1 November 1994 – We performed this study to determine whether perfused isolated human and rat hepatocytes have different sensitivities to anoxia‐reoxygenation injury. Oxygen free radicals were detected by lucigenin‐enhanced chemiluminescence. Lipid peroxidation was assessed by measuring malondialdehyde release. Cell injury was evaluated by measuring lactate dehydrogenase release and trypan blue uptake.

M. Sawkat Anwer – 1 November 1994 – Arginine vasopressin has been shown to activate the Na/H+ exchanger in hepatocytes by calcium/calmodulin‐dependent processes. Whether this activation also involves protein kinase C and is associated with changes in the intracellular pH setpoint was investigated in this study. Changes in pHi and intracellular Ca++ concentration were measured with the fluorescent probes BCECF and quin‐2, respectively. Intracellular pH recovery rate was calculated from timedependent changes in intracellular pH in hepatocytes acid‐loaded with sodium propionate.

Hiroaki Shimizu, Wei He, Ping Guo, Irena Dziadkoviec, Masaru Miyazaki, Rudolf E. Falk – 1 November 1994 – This study was designed to evaluate the use of serum hyaluronate as a marker of liver endothelial cell function after liver transplantation. We performed orthotopic liver transplantation in both isogeneic and allogeneic rejector models. After transplantation, hepatocyte function was assessed on the basis of serum ALT and total bilirubin levels, and liver endothelial cell function was judged on the basis of serum hyaluronate levels.

Kenneth Jensen, Christian Gluud – 1 November 1994 – Drawing on data from a previously published literature survey on the clinical and experimental epidemiology of the Mallory body, we discuss current theories on its development in a pro et contra manner. Conclusions have been largely left open to the interpretations of the reader because many are still speculative. The main results of this study characterize Mallory bodies as stereotypical histological byproducts to diverse hepatic injuries (mostly alcohol associated) of questionable pathogenic importance.

Alan F. Hofmann, James M. Crawford – 1 November 1994

Kirk Sperber – 1 November 1994

Gianfranco Tappero, Gioacchino Natoli, Giovanni Anfossi, Floriano Rosina, Francesco Negro, Antonina Smedile, Ferruccio Bonino, Alberto Angeli, Robert H. Purcell, Professor Mario Rizzetto, Massimo Levrero – 1 November 1994 – The intrahepatic accumulation of the c‐myc protooncogene product was observed on immunoflourescence in each of six patients with chronic hepatitis delta virus infection who exhibited the hepatitis D antigen in their livers. The c‐myc product was stained in the same nuclei that contained the hepatitis D antigen.