Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula

Antonio Francavilla, Thomas E. Starzl, Ken Porter, Carlo Scotti Foglieni, George K. Michalopoulos, Giuseppe Carrieri, Jose Trejo, Alessandro Azzarone, Michele Barone, Qi Hua Zeng – 1 October 1991 – Completely diverting portacaval shunt (Eck's fistula) in dogs causes hepatocyte atrophy, disruption of hepatocyte organelles, fatty infiltration and lowgrade hyperplasia. The effect of hepatic growth regulatory substances on these changes was assessed by constantly infusing test substances for four postoperative days after Eck's fistula into the detached left portal vein above the shunt.

Bone loss after liver transplantation

Jennifer A. McDonald, Colin R. Dunstan, Pamela Dilworth, Kenneth Sherbon, A. G. Ross Sheil, Richard A. Evans, Geoffrey W. McCaughan – 1 October 1991 – We studied 35 adult patients (mean age = 43 yr) referred for orthotopic liver transplantation. Spinal bone mineral density was measured by quantitative computed tomography scanning before transplantation (n = 35) and at 3 mo (n = 21) and 12 mo (n = 11) after orthotopic liver transplantation. The readings were corrected to age 50 yr, using the regression equations derived from normal control subjects.

Canine bile contains anticrystallization factors that inhibit precipitation of calcium carbonate

Lillian G. Dawes, Robert V. Rege, Edward W. Moore, Shuji Shimizu, J. Donald Ostrow – 1 October 1991 – Previous studies have strongly suggested that human bile contains a substance(s) that interferes with the precipitation of calcium phosphate and carbonate from solution. These studies, however, did not distinguish between calcium binding by biliary constituents resulting in decreased calcium carbonate saturation (alterations in solution thermodynamics) and true inhibition of calcium salt precipitation by kinetic factors.

Extracellular ATP, intracellular calcium and canalicular contraction in rat hepatocyte doublets

Tsuneo Kitamura, Ulrike Brauneis, Zenaida Gatmaitan, Irwin M. Arias – 1 October 1991 – Bile‐canaliculus contraction in rat hepatocyte doublets is postulated to involve activation of an actinmyosin system. We examined this hypothesis by determining the relationship between canalicular contraction and cystolic free Ca2+ ([Ca2+]i) concentration after extracellular addition of ATP or microdialysis of myosin light chain kinase or its Ca2+‐independent fragment, which retains catalytic activity.

Homogeneous distribution of phosphofructokinase in the rat liver acinus: A quantitative histochemical study

Wilma M. Frederiks, Frans Marx, Cornelis J. F. van Noorden – 1 October 1991 – A quantitative histochemical method was developed for the demonstration in rat liver of the activity of phosphofructokinase, one of the enzymes assumed to be rate‐limiting for glycolysis. The procedure was based on the reduction of a tetrazolium salt as final electron acceptor and a multistep reaction using the exogenous or endogenous auxiliary enzymes aldolase, triosephosphate isomerase and glyceraldehyde‐3‐phosphate dehydrogenase.

Efflux of protons from acidic vesicles contributes to cytosolic acidification of hepatocytes during ATP depletion

Steven F. Bronk, Gregory J. Gores – 1 October 1991 – The objective of this study was to determine the relationship between cytosolic pH and vesicular pH during ATP depletion. Using digitized video microscopy and single, cultured rat hepatocytes, cytosolic pH and vesicular pH were quantitated by ratio imaging of BCECF (2′, 7′ biscarboxyethyl‐5, 6‐carboxyfluorescein) fluorescence and fluoresceindextran fluorescence, respectively. Basal value for cytosolic pH was 7.26 and basal value for vesicular pH was 4.86.

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