Lack of immune potentiation by complexing HBsAg in a heat‐inactivated hepatitis B Vaccine with antibody in hepatitis B immunoglobulin

P. Nico Lelie, Peter J. A. J. van Amelsfoort, C. S. Martine de Groot, Ed Bakker, Wim Schaasberg, Jos C. M. Niessen, Henk W. Reesink – 1 July 1989 – In a randomized, dose‐response study among 305 health care workers, we examined whether the immunogenicity of a heat‐inactivated hepatitis B vaccine could be enhanced when HBsAg was complexed by anti‐HBs contained in hepatitis B immunoglobulin either at equivalent proportions or at 10‐fold antigen excess.

Hepatic enzymic zonation: A reevaluation of the concept of the liver acinus

Wouter H. Lamers, Aage Hilberts, Erwin Furt, Jerry Smith, Geertruida N. Jonges, Cornelis J. F. van Noorden, Jan Willem Gaasbeek Janzen, Robert Charles, Antoon F. M. Moorman – 1 July 1989 – The distribution pattern of a periportal enzyme (carbamoylphosphate synthetase) and a pericentral enzyme (glutamine synthetase) in human and rat liver has provided an objective parameter to delineate the zonal boundaries of the liver acinus.

Low dose of nitroglycerin failed to improve splanchnic hemodynamics in patients with cirrhosis: Evidence for an impaired cardiopulmonary baroreflex function

Richard Moreau, Dominique Roulot, Alain Braillon, Christophe Gaudin, Antoine Hadengue, Yannick Bacq, Didier Lebrec – 1 July 1989 – High doses of nitroglycerin may decrease portal pressure in patients with cirrhosis with untoward effects such as arterial hypotension and a decrease in systemic O2 uptake.

Characterization of the incorporation of woodchuck hepatitis virus surface antigen into hepatocyte plasma membrane in woodchuck hepatitis and in the virus‐induced hepatocellular carcinoma

Tomasz I. Michalak, Robert L. Snyder, Norma D. Churchill – 1 July 1989 – Interaction between woodchuck hepatitis virus surface antigen and proteins of hepatocyte plasma membranes were examined in the course of woodchuck hepatitis virus infection.

Acute massive necrosis of the liver induced by antibody/complement, not by T‐cells

Kunio Okuda – 1 July 1989 – Acute fulminant hepatic injury was induced in rats within 1 hour after a single intravenous injection of a monoclonal antibody to rat liver cell membrane (MoAb). The liver injury induced by MoAb was characterized by multiple focal hepa‐tocellular necrosis which was associated with marked increase in serum AST, ALT, and LDH activity. Inflammatory cell infiltration was not observed in the necrotic foci.

Similarities in maximal biliary bilirubin output in the normal rat after administration of unconjugated bilirubin or bilirubin diglucuronide

Andreas Sieg, Adolf Stiehl, Karel P. M. Heirwegh, Johan Fevery, Richard Raedsch, Burkhard Kommerell – 1 July 1989 – The rate‐limiting step in the overall plasma‐to‐bile transport of a saturating load of bilirubin is still a matter of controversy. We reassessed the apparent maximal biliary bilirubin excretion following i.v. infusion of unconjugated bilirubin and—for the first time—of highly purified bilirubin diglucuronide in the rat. The bilirubin diglucuronide preparation could be kept in a stable form at −20°C for at least 2 months after addition of 3 mM sodium ascorbate.

The effects of monensin on the transport of horseradish peroxidase into intracellular lumina in cultured rat hepatocytes

Imad Abou Hashieh, Lionel Rémy, Sylvie Mathieu, André Gérolami – 1 July 1989 – A kinetic study of horseradish peroxidase transport was carried out on short‐term cultured adult rat hepatocytes. After 4 hr of plating, cells were preincubated with monensin (1 μM) for 1 hr before their incubation with horseradish peroxidase for different times. Monensin treatment resulted in the dilatation of the Golgi apparatus and caused the appearance of numerous intracellular lumina lined with microvilli in associated as well as isolated hepatocytes but did not modify newly formed bile canaliculi.

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