Pathology of cytoskeleton of liver cells: Demonstration of mallory bodies (alcoholic hyalin) in murine and human hepatocytes by immunofluorescence microscopy using antibodies to cytokeratin polypeptides from hepatocytes
Helmut Denk, Werner W. Franke, Brigitte Dragosics, Ingrid Zeiler – 1 January 1981 – A cytoskeleton fraction enriched in intermediate‐sized filaments and resistant to high and low salt buffer containing Triton X‐100 was prepared from dissociated mouse liver cells and mouse liver homogenates. One‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis revealed a family of polypeptides in the relative molecular weight (Mr) range from 41,000 to 55,000, with two polypeptides (component A, Mr 55,000; component D, Mr 48,000) as major constituents.