Impaired autophagic clearance after cold preservation of fatty livers correlates with tissue necrosis upon reperfusion and is reversed by hypothermic reconditioning
Thomas Minor, Judith Stegemann, Andreas Hirner, Martina Koetting – 26 June 2009 – Fatty livers are particularly susceptible to mitochondrial alterations after cold preservation. We thus aimed to improve graft integrity by brief hypothermic oxygenation prior to warm reperfusion. Macrovesicular steatosis was induced in rat livers by fasting and subsequent feeding of a fat‐free diet enriched with carbohydrates. Fatty livers were retrieved and stored ischemically at 4°C for 20 hours in histidine‐tryptophan‐ketoglutarate solution.