Hepatitis C virus quasispecies in HIV‐infected women: Role of injecting drug use and highly active antiretroviral therapy (HAART)

Tomasz Laskus, Jeffrey Wilkinson, Roksana Karim, Wendy Mack, Marek Radkowski, Marina deGiacomo, Jonathan Nasseri, Zhi Chen, Jiaao Xu, Andrea Kovacs – 27 July 2007 – Despite the high frequency of HCV and HIV coinfection, little is known about HCV quasispecies in HIV‐positive patients. The current analysis included 236 HIV+/anti‐HCV+ women enrolled in the Women's Interagency HIV Study (WIHS). Hypervariable region 1 of the second envelope gene was analyzed by single‐strand conformation polymorphism (SSCP).

A modified international normalized ratio as an effective way of prothrombin time standardization in hepatology

Laurent Bellest, Valérie Eschwège, Raoul Poupon, Olivier Chazouillères, Annie Robert – 27 July 2007 – International Normalized Ratio (INR), which standardizes prothrombin time (PT) during oral anticoagulation, has been extended to standardize PT in liver diseases and is included in prognostic models such as the Model for End stage Liver Disease (MELD). However, mechanisms of PT prolongation in liver diseases differ from those involved in oral anticoagulation, and the thromboplastin reagents differ in their sensitivities to these 2 mechanisms.

Cytochrome P450IID6‐specific CD8 T cell immune responses mirror disease activity in autoimmune hepatitis type 2

Maria Serena Longhi, Munther J. Hussain, Dimitrios P. Bogdanos, Alberto Quaglia, Giorgina Mieli‐Vergani, Yun Ma, Diego Vergani – 27 July 2007 – Autoimmune hepatitis type 2 (AIH‐2) is a severe organ‐specific disorder characterized by liver kidney microsomal antibody type 1 targeting cytochrome P4502D6 (CYP2D6). Growing evidence implicates the involvement of CD8 T cell immune responses in its pathogenesis. We investigated CYP2D6‐specific CD8 T cell human leukocyte antigen (HLA)‐A2 restricted responses in AIH‐2 (20 patients, 11 HLA‐A2+).

The international normalized ratio calibrated for cirrhosis (INRliver) normalizes prothrombin time results for model for end‐stage liver disease calculation

Armando Tripodi, Veena Chantarangkul, Massimo Primignani, Federica Fabris, Alessandra Dell'Era, Cinzia Sei, Pier Mannuccio Mannucci – 27 July 2007 – The model for end‐stage‐liver‐disease (MELD) is a mathematical score used to prioritize patients for liver transplantation and includes results for creatinine, bilirubin, and prothrombin time (PT) expressed as international normalized ratio (INR).

Noninvasive diagnosis and monitoring of nonalcoholic steatohepatitis: Present and future

Anna Wieckowska, Arthur J. McCullough, Ariel E. Feldstein – 27 July 2007 – Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the United States, and its prevalence is increasing worldwide. It currently affects approximately 30% of adults and 10% of children in the United States. NAFLD represents a wide spectrum of conditions ranging from simple fatty liver which in general follows a benign nonprogressive clinical course, to nonalcoholic steatohepatitis (NASH), which is a more serious form of NAFLD that may progress to cirrhosis and end‐stage liver disease.

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