P Invernizzi, A Crosignani, P M Battezzati, G Covini, G De Valle, A Larghi, M Zuin, M Podda – 30 December 2003 – Reports from North America and Northern Europe have suggested that antimitochondrial antibody (AMA) negative primary biliary cirrhosis (PBC) is a distinct chronic cholestatic liver disease with high prevalence of serum non‐organ‐specific autoantibodies other than AMA.

A Tanaka, S Iwabuchi, M Takatori, A Ohno, H Yamada, N Hashimoto, Y Ikeda, T Kato, K Nishioka, S Iino, K Yamamoto – 30 December 2003 – The immunopathogenesis of autoimmune hepatitis (AIH), and the role of T cells in the onset and maintenance of this disease, are still unclear. Since T cells expand clonally after stimulation by an antigen, it is important to analyze the behavior of T cells at a clonal level.

N Miyanari, Y Yamaguchi, K Matsuno, A Tominaga, M Goto, O Ichiguchi, K Mori, M Ogawa – 30 December 2003 – A single intravenous injection of freshly heparinized blood from a donor‐specific blood transfusion (DST) seven days before transplantation significantly prolongs the subsequent survival of hepatic allografts from ACI(RT1a) to LEW(RT11) rats. We used W3/25 (anti‐CD4) and OX22 (anti‐CD45 RC: an isoform of leukocyte‐common antigen [CD45R]) monoclonal antibodies to investigate the cellular identity of hepatic allograft infiltrates following transplantation.

L Aloj, R E Carson, L Lang, P Herscovitch, W C Eckelman – 30 December 2003 – We have evaluated the use of [18F]holo‐transferrin ([18F]Tf) and positron emission tomography (PET) to measure in vivo Tf receptor expression and recycling using the baboon liver as a model. [18F]Tf was intravenously injected in three baboons and dynamic PET was performed over the region containing liver and spleen. In two of the three baboons, [18F]albumin ([18F]Alb), labeled with the same technique, was administered 3 hours later. Time activity curves (TACs) were obtained from liver and spleen for both tracers.

P Caraceni, H S Ryu, V Subbotin, N De Maria, A Colantoni, L Roberts, F Trevisani, M Bernardi, D H Van Thiel – 30 December 2003 – The aim of this study was to determine whether rat hepatocytes isolated from steatotic or nonsteatotic livers have different thresholds for injury due to anoxia‐reoxygenation. Rats were fed ethanol or control diets for 8 weeks. Histology showed that more than 75% of the hepatocytes in alcohol‐fed and less than 3% in control animals contained fatty vacuoles. The glycogen content was significantly reduced in steatotic livers.

C Peralta, G Hotter, D Closa, E Gelpí, O Bulbena, J Rosello‐Catafau – 30 December 2003 – The effects of ischemic preconditioning on rat liver integrity, as well as the implication of nitric oxide (NO) and adenosine in this process, has been evaluated. Preconditioning before ischemia‐reperfusion prevented the increases in alanine transaminase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) levels, but did not modify blood flow.

P A McCormick, L Greenslade, C C Kibbler, J K Chin, A K Burroughs, N McIntyre – 30 December 2003 – Aminoglycosides are frequently used to treat sepsis in patients with liver disease. However, it has been suggested that cirrhotic patients are particularly sensitive to aminoglycoside‐induced renal dysfunction. We investigated the efficacy and incidence of renal impairment with netilmicin plus mezlocillin compared with ceftazidime in 128 cirrhotic patients who required empirical treatment for sepsis.

S Matsuzaki, M Onda, T Tajiri, D Y Kim – 30 December 2003 – We studied the hepatic functional reserve in the lobes of the liver in 28 patients with chronic liver disease and 13 controls using single photon emission computed tomography (SPECT) imaging with a radiolabeled asialoglycoprotein analog, Technetium‐99m‐diethylenetriaminepentaacetic acid‐galactosyl‐human serum albumin (Tc‐99m GSA).

C K Leow – 30 December 2003

H Cheng, N S Khairullah – 30 December 2003