Onni Nlemelä, Seppo Parkkila, Seppo Ylä‐herttuala, Jesus Villanueva, Boris Ruebner, Charles H. Halsted – 1 October 1995 – The pathogenesis of alcohol‐induced liver disease involves the adverse effects of ethanol metabolites and oxidative tissue injury. Previous studies indicated that covalent protein adducts with reactive aldehydes may be formed in alcohol consumers.
Thomas Leitha, Anton Staudenherz, Marcela Hermann, Manfred Hüttinger, Bernhard Gmeiner – 1 October 1995 – This study quantifies the parenchymal and nonparen‐chymal uptake of technetium‐99m (99mTc)‐ and indium‐III (111In)‐low‐density lipoprotein (LDL) in different states of hepatic LDL‐receptor activity to validate quantitative LDL scintigraphy. Iodine‐125 (125I)‐LDL was used as reference tracer.
Rudolf A. Heijtink, Johannes Kruining, Yvonne A. M. Weber, Robert A. de Man, Solko W. Schalm – 1 October 1995 – Two human monoclonal antibodies with anti‐hepatitis B activity were investigated separately and as a mixture by means of an “inhibition in solution” assay. With this assay the capacity of anti‐HBs antibodies to inhibit the binding of hepatitis B surface antigen (HBsAg) with solid‐phase anti‐HBs (Ausria II, Abbott Laboratories) was studied. Both HBsAg of different subtypes and purified Dane particles were used.
Howard N. Sankary, Anita Chong, Preston Foster, Esther Brown, Jikun Shen, Robert Kimura, Guarimella Rayudu, James Williams – 1 October 1995 – Data from recent studies suggest that donor fasting imparts a beneficial effect on the viability of transplanted hepatic allografts. Because starvation may temporarily inactivate Kupffer cells, and because these cells are the likely mediators of liver injury after prolonged preservation‐reperfusion, the purpose of this study is to establish a link between improved organ viability and Kupffer cell inactivation caused by donor allograft fasting.
John C. Hoefs, Felix Wang, Gary Kanel, Philip Braunstein – 1 October 1995 – Sulfur colloid distribution on liver‐spleen scan is determined by the perfused Kupffer cell mass. The perfused Kupffer cell mass is proportional to the perfused hepatocyte mass, but is less affected by acute changes in hepatocyte function. Thus, sulfur colloid distribution parameters (precisely measured by quantitative liver‐spleen scan [QLSS]) may be an excellent test of the perfused hepatic mass.
Francis K. L. Chan, Yikun Zhang, Francis R. Sutherland, Eldon A. Shaffer – 1 October 1995 – Altered hepatic secretory function after orthotopic liver transplantation constitutes a major perioperative clinical problem. Cholestasis and cholesterol gallstone formation are among the most frequent complications reported. Such changes in the allograft secretory function can be secondary to many factors like graft injury due to preservation and marked rejection, surgical complications, immunosuppressive therapy, and sepsis.
América Giménez, Joaquim Hostench, Stamatis C. Stamatoglou, Carlos Enrich – 1 October 1995 – We have studied the distribution and expression of laminin during rat liver regeneration by immunofluorescence and immunoblotting using affinity‐purified laminin antibodies. Laminin was localized on sinusoidal surfaces in normal and regenerating hepatic parenchyma, but enhanced expression was detected during regeneration from 6 hours to 7 days after a partial hepatectomy.
Seiichiro Kamimura, Hidekazu Tsukamoto – 1 October 1995 – Kupffer cell‐derived cytokines are believed to play pivotal paracrine roles in the pathogenesis of alcoholic liver disease (ALD). To evaluate this hypothesis, Kupffer cell gene expression of tumor necrosis factor‐alpha (TNFα), interleukin (IL)‐6, and transforming growth factor‐beta 1 (TGFβ1) were directly examined in the rat model of ALD. Kupffer cells were isolated from the model after 10 and 17 weeks of intragastric ethanol infusion. These two durations resulted in focal hepatocellular injury and liver fibrogenesis, respectively.