David E. Milov, Wou‐Seok Jou, Rachel B. Shireman, Paul W. Chun – 1 February 1992 – Bile salts are potent inhibitors of bovine carbonic anhydrase and human carbonic anhydrase I and human carbonic anhydrase II. To further characterize the binding of bile salts to carbonic anhydrase, rate constants for the CO2 hydration reaction in the presence of deoxycholate, cholate, glycocholate and taurocholate were determined using stop‐flow experments.
Sun‐Mee Lee, Mark G. Clemens – 1 February 1992 – This study was done to determine the relationship between microsomal lipid peroxidation during hepatic ischemia/reperfusion and alteration in cytochrome P‐450–dependent drug metabolism. Rats were pretreated with α‐tocopherol to inhibit lipid peroxidation or with vehicle (soybean oil) and then subjected to 60 min no‐flow hepatic ischemia in vivo. Control animals were time‐matched sham‐ischemic animals. After 1, 5 or 24 hr of reperfusion, liver microsomes were isolated and cytochrome P‐450 and mixed function oxidases were studied.
J. Michael Henderson, Gregory J. Mackay, Michael Hooks, Judith L. Chezmar, John R. Galloway, Thomas F. Dodson, Michael H. Kutner – 1 February 1992 – This study measured cardiac output before and 1 or 2 yr after orthotopic liver transplantation in 23 patients. Cardiac output was measured by thermodilution before transplantation and by first‐pass radionuclide angiocardiography at follow‐up.
Hiroaki Okamoto, Fumio Tsuda, Atsuhiko Machida, Eisuke Munekata, Yoshihiro Akahane, Yoshiki Sugai, Kazuo Mashiko, Takehiro Mitsui, Takeshi Tanaka, Yuzo Miyakawa, Makoto Mayumi – 1 February 1992 – Immunoassays were developed to detect antibodies against oligopeptides deduced from the putative core gene of hepatitis C virus, and their performances were compared with that of the commercial immunoassay for antibodies against the product of nonstructural regions of hepatitis C virus (anti‐C100‐3).
1 February 1992
Agata Budkowska, Pascal Dubreuil, Thierry Poynard, Patrick Marcellin, Marie‐Anne Loriot, Patrick Maillard, Jacques Pillot – 1 January 1992 – Serial sera were collected prospectively during the clinical course of 13 HBsAg carriers with chronic liver disease and analyzed for ALT levels, pre‐S1 and pre‐S2 antigens and corresponding antibodies and other serological hepatitis B virus markers.
Tomoaki Tomiya, Sumiko Nagoshi, Kenji Fujiwara – 1 January 1992 – Serum human hepatocyte growth factor levels were measured using a newly developed enzyme‐linked immunosorbent assay kit in patients with liver diseases. Serum human hepatocyte growth factor levels were increased in correlation with derangements of prothrombin time, total bilirubin and other parameters reflecting hepatocellular dysfunction in 112 patients with chronic liver disease. The levels were positively correlated with serum AST and ALT levels in 59 of these patients whose prothrombin times were within the normal range.
M. Sawkat Anwer, James M. Atkinson – 1 January 1992 – The effect of Ca+ + mobilizing agonists arginine vasopressin and phenylephrine on Na+/H+ exchange was studied in freshly isolated hepatocytes and isolated perfused rat livers. The activity of Na+/H+ exchange was determined from the rate of H+ efflux, 22Na uptake and pHi recovery. Arginine vasopressin and phenylephrine stimulated H+ efflux and 22Na uptake in isolated rat hepatocytes and increased the rate of pHi recovery from acid‐loaded hepatocytes. These effects were inhibited by amiloride.
Samuel S. Lee, Kaj Johansen, Didier Lebrec – 1 January 1992 – We studied the hemodynamics in four groups of rats with combinations of mesenteric hypertension and portal diversion. Operations created three groups with mesenteric hypertension and different degrees of portal venous diversion: mesenteric vein stenosis, portal vein stenosis and end‐to‐side portacaval anastomosis with mesenteric vein stenosis, the fourth group had only portacaval anastomosis. A control group had sham operations.
Alain Braillon, Christophe Gaudin, Jorge L. Poo, Richard Moreau, Bertrand Debaene, Didier Lebrec – 1 January 1992 – In patients with cirrhosis, the significance of elevated plasma catecholamine concentrations is unclear. Thus we investigated the relationship between plasma catecholamine concentrations and the hemodynamic effect of pindolol (an index of sympathetic vascular tone) in 10 patients with cirrhosis.