Localization of the heme‐binding protein in the cytoplasm and of a heme‐binding protein‐like immunoreactive protein in the nucleus of rat liver parenchymal cells: Immunocytochemical evidence of the subcellular distribution corroborated by radioimmunoassay

H. Dariush Fahimi, Alfred Voelkl, Styliani H. Vincent, Ursula Muller‐Eberhard – 1 May 1990 – The abundant heme‐binding protein of the liver, probably identical with Z‐protein or liver fatty acid‐binding protein, has an apparent molecular weight of 14,000 Da and is presumably involved in the intracellular transport of a variety of compounds. The cellular and subcellular distribution of HBP in the liver was studied in adult male and female rats by postembedding immunocytochemistry using the protein A‐gold technique.

The formation of bile canaliculi in human hepatoma cell lines

Jen‐Hwey Chiu, Cheng‐Po Hu, Wing‐Yiu Lui, Szecheng J. Lo, Chungming Chang – 1 May 1990 – Hepatocytes, known as polarized epithelial cells, are composed of sinusoid, basolateral and bile canalicular domains. Each domain contains proteins specific for it. Our studies indicate that the well‐differentiated human hepatoma cell lines HepG2 and HuH‐7 formed bile canaliculi in tissue culture, whereas the poorly differentiated hepatoma cell lines HA22T/VGH and SK‐HEP‐1 did not.

Formation of biliary thrombi in protoporphrin‐induced cholestasis in perfused rat liver

Malcolm M. Berenson, Cathy Gunther, Wade S. Samowitz, David J. Bjorkman – 1 May 1990 – The effect of bile acids on the formation of biliary thrombi in protoporphyrin‐induced cholestasis was determined by perfusing isolated rat livers with taurocholate, chenodeoxycholate and ursodeoxycholate with and without protoporphyrin. Protoporphyrin‐induced reduction of bile flow was similar in the presence of each bile acid.

The ursodeoxycholate dose‐dependent formation of ursodeoxycholate‐glucuronide in the rat and the choleretic potencies

Hajime Takikawa, Naoyo Sano, Tohru Narita, Masami Yamanaka – 1 May 1990 – The reason for the discrepancy between bile flow and biliary bile acid excretion during ursodeoxycholate infusion in rats is unknown. We found that ursodeoxycholate‐glucuronide is formed during ursodeoxycholate infusion at higher doses. Ursodeoxycholate infusion (1 to 3 μmol/min/100 gm body weight) for 90 min caused marked hypercholeresis, and the previously reported discrepancy between bile flow and biliary bile acid excretion was observed when bile acid concentrations were measured by regular enzymatic methods.

Endothelial cell transformation in primary biliary cirrhosis: A morphological and biochemical study

Christopher Babbs, Najib Y. Haboubi, Jonathan M. Mellor, Alexander Smith, Brenda P. Rowan, Thomas W. Warnes – 1 May 1990 – There is increasing interest in the changes of the endothelial lining of the hepatic sinusoids during the development of chronic liver disease. In this study we looked for evidence of hepatic sinusoidal endothelial cell transformation and basement membrane production in patients with primary biliary cirrhosis.

Cholesteryl ester storage disease: Hepatopathology and effects of therapy with lovastatin

Adrian M. Di Bisceglie, Kamal G. Ishak, Lionel Rabin, Jeffrey M. Hoeg – 1 May 1990 – We describe three patients with cholesteryl ester storage disease. Diagnosis was confirmed by demonstrating a deficiency in lysosomal acid cholesteryl hydrolase activity in cultured skin fibroblasts from each of these patients. All had hepatomegaly, elevated serum aminotransferase activities and hyperlipoproteinemia. Histological examination of liver biopsy specimens before treatment revealed accumulation of fat within hepatocytes, bile duct epithelium and endothelial and Kupffer cells.

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