Isolated rat hepatocyte couplets in short‐term culture: Structural characteristics and plasma membrane reorganization

Anil Gautam, Oi‐Cheng Ng, James L. Boyer – 1 March 1987 – Studies of canalicular bile secretion have been limited due to lack of direct access to the canalicular lumen. Isolated rat hepatocyte couplets, consisting of two hepatocytes enclosing a canalicular space, have been proposed as a primary secretory unit that may be useful for direct studies of unmodified canalicular bile secretion. The present study examines their structural characteristics and plasma membrane reorganization.

Prevention of recurrent bleeding in cirrhotics with recent variceal hemorrhage: Prospective, randomized comparison of propranolol and sclerotherapy

Wolfgang E. Fleig, Eduard F. Stange, Roland Hunecke, Wolfgang Schönborn, Ulrike Hurler, Kordula Rainer, Wilhelm Gaus, Hans Ditschuneit – 1 March 1987 – To compare the efficacy of endoscopic paravariceal sclerotherapy and oral propranolol in the prevention of recurrent upper gastrointestinal bleeding, 78 cirrhotic patients were randomly assigned to either treatment after an endoscopically proven bleed from esophageal varices.

Long‐term follow‐up study of asymptomatic hbsag‐positive voluntary blood donors in austria: A clinical and histologic evaluation of 242 cases

Brigitte Dragosics, Peter Ferenci, Eva Hitchman, Helmut Denk – 1 March 1987 – Two hundred forty‐two voluntary blood donors, referred after detection of HBsAg positivity, underwent clinical evaluation and liver biopsy and were prospectively followed for an average of 3.5 years. At initial testing, 65% of HBsAg carriers had normal laboratory findings; during follow‐up, 26% of these carriers developed abnormal test results, at least transiently. Liver histology was normal in 31.4%, revealed nonaggressive liver disease in 63.6% and chronic active hepatitis or cirrhosis in 5%, only.

β2‐Adrenergic receptors in propranolol therapy: Does down‐regulation do it?

Andrew K. Burroughs – 1 March 1987 – The density and affinity of beta‐2‐adrenoceptors on mononuclear cells from peripheral blood were studied in fifteen patients with cirrhosis of different severity and in thirteen controls. There was no significant difference between cirrhotic patients and controls in density or affinity of beta‐2 binding sites. Within the cirrhotic group, however, the number of binding sites per cell was significantly lower in patients with severe ascites than in patients with mild to moderate or no ascites.

Influence of branched‐chain amino acids and branched‐chain keto acids on protein synthesis in isolated hepatocytes

Wolfgang Base, Carl Barsigian, Alisa Schaeffer, Ellen Shaw, Jose Martinez, Willis C. Maddrey – 1 March 1987 – We investigated the effects of the branched‐chain amino acids—valine, leucine and isoleucine—or their keto analogs, the branched‐chain keto acids—α‐ketoisovaleric acid, α‐ketoisocaproic acid and α‐keto‐β‐methylvaleric acid—on protein synthesis and secretion by monolayers of rabbit hepatocytes incubated with [35S] methionine in pulse‐chase and steady‐state experiments.

Toxic effects of the photoproducts of chlorpromazine on cultured hepatocytes

José V. Castell, M. A. José Gómez‐Lechón, Miguel A. Miranda, Isabel M. Morera – 1 March 1987 – The photodegradation of chlorpromazine, a drug frequently used in psychotherapy, was examined under different sets of experimental conditions. A primary culture of rat hepatocytes was used to evaluate the possible hepatotoxicity of the chlorpromazine photoproducts, keeping in mind the following criteria: leakage of cytosolic enzymes; attachment index to culture plates, and albumin synthesis.

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