William W. Faloon – 1 July 1984

Ardythe A. Mccracken, Merrill Emmett, Alfred J. Crowle, Jerry L. Brown – 1 July 1984 – We have used crossed Immunoelectrophoresis to identify and establish the relative amounts of serum proteins secreted by a differentiated cell line (Fao) derived from a Reuber H35 rat hepatoma. Our results show that these cells secrete at least 15 plasma proteins. Ten of these: albumin, α1‐antitrypsin, α1‐lipoprotein, α1‐macroglobulin, α1‐antichymotrypsin, GC‐globulin (transcalciferin), fibronectin, hemopexin, transferrin and the C3 component of complement have been identified.

Yukio Yokoi, Toshihiko Namihisa, Hlroyuki Kuroda, Ikutoshi Komatsu, Akihisa Miyazaki, Sumio Watanabe, Koh Usui – 1 July 1984 – Desmin is a smooth muscle protein consisting of intermediate filaments. It is also present in other cell types. We obtained desmin antibodies from New Zealand white rabbits which were immunized with desmin following its extraction from chicken gizzard smooth muscle. By immunocytochemical methods, the antibody stained the Z‐line of rat skeletal myofibrils and produced only one band with chicken gizzard desmin by immunoblotting.

Bruno Descos, Olivier Bernard, Francis Brunelle, Jacques Valayer, Delphine Feldmann, Michelle Hadchouel, Daniel Alagille – 1 July 1984 – Ten infants of less than 6 months of age presented with cholestatic jaundice and gallstones. Jaundice occurred after a lag period, and sepsis was present in three children. Ultrasound examination showed dilatation of intrahepatic and extrahepatic bile ducts in eight patients and detected cholelithiasis in three.

Yun‐Fan Liaw, Miau‐Ju Huang, Chia‐Ming Chu, I‐Shyan Sheen, Deng‐Yn Lin – 1 July 1984 – To examine the period between disappearance of hepatitis B e antigen (HBeAg) and appearance of anti‐HBe, 48 patients with clinicopathologically verified chronic B hepatitis were followed every 1 to 3 months after HBeAg clearance. Sera were tested by radioimmunoassay for HBeAg and anti‐HBe. Anti‐HBe appeared in days to years, mostly (78.7%) within 1 year, after disappearance of HBeAg. Only 40.5% of patients had an “e‐window” shorter than 1 month.

1 May 1984

1 May 1984

Oscar W. Portman, Manfred Alexander, Charles E. Cornelius, Jayanta Roy Chowdhury, Namita Roy Chowdhury, Irwin M. Arias – 1 May 1984 – Bolivian squirrel monkeys (Saimiri sciureus) have fasting unconjugated hyperbilirubinemia (males: 2.0 ± 0.14; females: 3.0 ± 0.26 mg per dl) which resembles that of humans with Gilbert's syndrome. Closely related Brazilian squirrel monkeys have fasting levels (males: 0.29 ± 0.045; females: 0.36 ± 0.073 mg per dl) similar to normal people. The purpose of this study was to identify the underlying mechanisms and the nutritional factors involved.

Dale C. Snover, Charles A. Horwitz – 1 May 1984 – Six liver biopsies from previously healthy adult patients with cytomegalovirus (CMV) mononucleosis were studied by routine light microscopy and by the immunoperoxidase technique for CMV antigen. Light microscopical findings consisting of a mononuclear portal and sinusoidal infiltrate, increased hepatocellular mitotic activity and minimal hepatocellular necrosis were consistently found. Less common features were granuloma formation and bile duct epithelial damage.

Bruno Clement, Christiane Guguen‐Guillouzo, Jean‐Pierre Campion, Denise Glaise, Michel Bourel, Andre Guillouzo – 1 May 1984 – High yields of viable human hepatocytes were obtained by enzymatic perfusion of the left hepatic lobe of kidney donors and cultured alone or with an epithelial cell line derived from rat liver. In conventional cultures, human hepatocytes did not survive more than 2 to 3 weeks and by Day 8 decreased their ability to secrete albumin. When co‐cultured, they survived for more than 2 months and secreted high levels of albumin even in a serum‐free medium.