D. Brian Jones, George W. Mihaly, Richard A. Smallwood, Lorraine K. Webster, Denis J. Morgan, Norman P. Madsen – 1 May 1984 – Although the adverse effect of hypoxia on drug metabolism is well documented in subcellular systems, its effect on drug clearance by the intact liver has not been defined. This study was undertaken in the isolated perfused rat liver to examine the effects of acute hypoxia on the hepatic elimination of two highly cleared substances‐propranolol and sodium taurocholate. Hypoxia was established by equilibrating the perfusate with 100% nitrogen rather than 100% oxygen.

A. De Margreet Leeuw, Séan P. Mccarthy, Albert Geerts, Dick L. Knook – 1 May 1984 – Primary cultures and cell lines were established from suspensions of purified fat‐storing cells isolated from the rat liver. When seeded at a suitable density, fat‐storing cells in primary culture reached confluency in 3 to 4 days and could be transferred and established as cell lines for at least two passages. The typical morphological characteristics of fat‐storing cells in vivo were retained in the cells during primary culture.

Ahmed E. O. Zaki, Roland J. Ede, Michael Davis, Roger Williams – 1 May 1984 – Permeability of the blood brain barrier in relation to the development of hepatic encephalopathy was investigated in two animal models of acute hepatic failure, in one of which there was the potential for recovery (D‐galactosamine‐induced hepatitis). In both this and the hepatic devascularization model, there was an approximate 3‐fold increase in the passive permeability of the blood brain barrier to inulin and sucrose.

1 May 1984

Shunzaburo Iwatsuki – 1 May 1984

Ki M. Mak, Charles S. Lieber – 1 May 1984 – The effects of chronic alcohol consumption on the ultrastructure of endothelial fenestrations in liver sinusoids were studied by scanning electron microscopy in surgical liver biopsies of 16 baboons pair‐fed with nutritionally adequate diets containing alcohol or isocaloric carbohydrate for up to 112 months. Alcohol consumption for 4 to 24 months resulted in a decreased number of fenestrations (1.4 per μm2 of the endothelial surface vs. 3.3 in pair‐fed controls; p < 0.01) and an increase in their geometric mean diameter (115.6 vs.

Charles S. Davidson, Vivian E. Shih – 1 May 1984

Marisabel Mourelle, Marcos Rojkind – 1 May 1984 – When compared to normal liver membranes, purified plasma membranes of regenerating liver and Morris hepatomas contain low but variable capacities to bind glucagon. This property is inversely related to the capacity of the isolated hepatocytes to bind to heterologous biomatrix glycoproteins. Since these parameters are characteristic of the proliferative state of the cells, it was important to further study the glucagon receptor protein and stimulation of adenylate cyclase activity.

Kwesi N. Tsiquaye, Tim J. Harrison, Bernard Portmann, Shanlian Hu, Arie J. Zuckerman – 1 May 1984 – Two hepatitis B virus carrier chimpanzees which were superinfected with hepatitis A virus developed acute hepatitis followed by the production of antibodies to hepatitis A virus. The Southern blot technique employed to monitor liver hepatitis B virus DNA revealed that the amount of viral DNA in both animals was significantly reduced during the acute phase of hepatitis A infection. The levels of plasma hepatitis B DNA polymerase activity were also reduced in one chimpanzee.

D. Paul Cleland, T. Carl Bartholomew, Barbara H. Billing – 1 May 1984 – The effect of bile duct ligation for 5 days on the hepatic transport of sulfated and nonsulfated bile acids was studied. Tracer doses of radioactive bile acids [3H]taurochenodeoxycholate‐3‐sulfate [3H]chenodeoxycholate‐3‐sulfate, [3H]taurochenodeoxycholic acid and [14C]taurocholic acid were injected 90 min after relief of obstruction when the plasma total bile acid concentration had reverted to normal.