Rapid, large‐scale formation of porcine hepatocyte spheroids in a novel spheroid reservoir bioartificial liver
Scott L. Nyberg, Joseph Hardin, Bruce Amiot, Upendra A. Argikar, Rory P. Remmel, Piero Rinaldo – 20 July 2005 – We have developed a novel bioreactor based on the observation that isolated porcine hepatocytes rapidly and spontaneously aggregate into spheroids under oscillation conditions. The purpose of this study was to characterize the influence of oscillation frequency (0.125 Hz, 0.25 Hz), cell density (1‐10 × 106 cells/mL), and storage condition (fresh, cryopreserved) of porcine hepatocytes on the kinetics of spheroid formation.