Cheng Qian, Roberto Bilbao, Oscar Bruña, Jesús Prieto – 1 July 1995 – We have analyzed the ability of a recombinant replication‐defective adenovirus to transfer the thymidine kinase gene of herpes simplex virus (HSV‐tk) into hepatocellular carcinoma (HCC) cells to confer sensitivity to ganciclovir. Three HCC cell lines (Hep3B, PLC/PRF/5, and HepG2) were efficiently infected in vitro by a recombinant adenovirus carrying lacZ reporter gene (Ad‐CMVlacZ).

Mario Pirisi, Carlo Fabris, Giorgio Soardo, Edmondo Falleti, Daniele Gasparini, Daniela Vitulli, Pierluigi Toniutto, Fabio Gonano, Ettore Bartoli – 1 July 1995 – It has been shown previously that erythropoietin expression in vitro by hepatoma cells increases in response to hypoxia. To verify whether hypoxia of the tumor might result in hepatic release of erythropoietin in vivo, serum erythropoietin concentrations were measured immunoenzymatically in 12 patients (5 women, 7 men) who underwent transarterial chemoembolization for hepatocellular carcinoma.

Michael Roden, Martin Prskavec, Clemens Fürnsinn, Ibrahim Elmadfa, Jürgen König, Barbara Schneider, Oswald Wagner, Werner Waldhäusl – 1 July 1995 – Selenium, an essential trace element, has been shown to decrease plasma glucose concentrations of diabetic rats. To study the short‐term effects of selenium on hepatic carbohydrate metabolism, isolated perfused livers of fed Sprague‐Dawley rats were continuously infused with sodium selenite for 90 minutes.

Akira Nakano, David L. Marks, Pamela S. Tietz, Piet C. De Groen, Nicholas F. Larusso – 1 July 1995 – The turnover rate of an individual protein is a function of the rates of synthesis and loss of that protein. For most intracellular proteins, loss occurs through digestion by lysosomal or cytosolic proteases. Although a significant proportion of hepatic lysosomal enzymes is released from the hepatocyte by excretion into bile, the contribution of biliary excretion to the turnover of hepatic lysosomal enzymes has never been measured.

Yoshiyuki Miwa, Masahiko Kato, Hisataka Moriwaki, Masataka Okuno, Jun'ichi Sugihara, Horoo Ohnishi, Takashi Yoshida, Yasutoshi Muto, Mitsuo Nakayama, Yujiro Morioka, Kouzou Asagi – 1 July 1995 – Branched‐chain amino acids (BCAA) are known to improve hepatic encephalopathy as well as protein malnutrition in cirrhosis. However, such effects in acute hepatic failure (AHF) remain to be elucidated. The current study was conducted to investigate whether BCAA improves protein metabolism in AHF.

Paul J. Winwood, Detlef Schuppan, John P. Iredale, Choudhury A. Kawser, Andrew J. P. Docherty, Michael J. P. Arthur – 1 July 1995 – Release of 92‐kd type IV collagenase/gelatinase, also known as gelatinase B, by inflammatory and tumor cells is increasingly recognized and is believed to facilitate cellular migration across basement membranes. It has been implicated in the pathogenesis of many diseases, but little is known of its cellular origin(s) and function in liver.

Caroline A. Riely, Alexander S. Petrides – 1 July 1995

Matthias Wettstein, Birgitta Noé, Dieter Häussinger – 1 July 1995 – The influence of endotoxin on the hepatic metabolism and elimination of <3>H‐leukotriene C4 (LTC4) and <3>H‐leukotriene E4 was studied in the single‐pass perfused rat liver. Endotoxin (4 mg/kg body mass) was injected intraperitoneally 8 to 10 hours before livers were isolated for perfusion. Tritiated leukotriene C4 and leukotriene E4 (10 nmol/L) were infused for 5 minutes, and metabolites in bile were determined by high‐pressure liquid chromatography.

John J. Fung, Jorge Rakela, Antonio Pinna, Satoru Todo – 1 July 1995

David R. Gretch, Carlos E. Bacchi, Lawrence Corey, Corazon Dela Rosa, Richard R. Lesniewski, Kris Kowdley, Allen Gown, Indra Frank, James D. Perkins, Robert L. Carithers – 1 July 1995 – We report a prospective clinical and virological study of 18 patients undergoing orthotopic liver transplantation, selected because of hepatitis C virus (HCV) RNA positivity before transplantation. Nine of the 18 patients (50%) developed chronic active hepatitis (CAH) in liver allografts during the first year posttransplantation; hepatitis was first observed between 6 and 25 weeks post‐transplantation.