Identification and characterization of liver nonparenchymal cells by flow cytometry
Mark Doolittle, Roger Bohman, Andres Durstenfeld, Joseph Cascarano – 1 July 1987 – Our objective was to correlate biochemical or functional properties that could be measured by fluorescence to individual nonparenchymal cell types by the simultaneous detection of forward angle light scatter and fluorescence. Cells were released from liver following selective digestion of hepatocytes by Pronase perfusion. To fractionate the cells according to size, isolates were subjected to unit gravity sedimentation on a 1 to 5% sucrose gradient.