Correspondence
Leslie Zieve – 1 May 1987
The in vitro production of Dane‐like particles: Is it live or memorex?
Francis A. Farraye, Jerome B. Zeldis – 1 May 1987 – Closed‐circular HBV DNA was introduced into cells of the established human hepatoma culture HepG2. The culture medium of one of 40 single‐cell clones contained HBV surface antigen (HBsAg), core‐related antigens (HBc/eAg), and HBV DNA sequences. HBV DNA and DNA polymerase activity were detected in particles resembling both nucleocapsids and complete virions (Dane particles). Intracellular integrated and extrachromosomal HBV DNA sequences were detected.
Establishment and characterization of a human combined hepatocholangiocarcinoma cell line and its heterologous transplantation in nude mice
Tastuo Murakami, Hirohisa Yano, Masafumi Maruiwa, Shigetaka Sugihara, Masamichi Kojiro – 1 May 1987 – A human cell line (KMCH‐1) derived from a surgical specimen of combined hepatocellular and cholangiocarcinoma has been established. The original tumor consisted of both hepatocellular carcinoma of the trabecular type and cholangiocellular carcinoma. This cell line has been maintained for 26 months through 75 passages. KMCH‐1 cells show characteristics of adenocarcinoma on light and electron microscopy. They proliferate in culture in a pave stone arrangement.
Secretory component‐dependent binding of immunoglobuin A in the rat, monkey and human: A comparison of intestine and liver
Christopher K. Daniels, Douglas L. Schmucker – 1 May 1987 – The source and significance of immunoglobulin A in bile remains controversial. In the rat, and several other species, immunoglobulin A is transported through hep‐atocytes by a specific receptor, secretory component. In humans, immunohistochemical methods have indicated a distinct lack of receptors for immunoglobulin A on hepatocytes. Binding assays with 125I‐immunoglobulin A and membranes from hepatocytes and intestinal cells of the rat display secretory component‐dependent binding.
Characterization of ciprofibrate and clofibric acid as peroxisomal proliferators in primary cultures of rat hepatocytes
Dennis R. Feller, Yogendra Singh, Vilas R. Shirhatti, Thomas A. Kocarek, Cuo‐Tung Liu, Gopal Krishna – 1 May 1987 – We have determined the comparative activities of peroxisomal proliferators, ciprofibrate and clofibric acid on various hepatic parameters associated with endoplasmic reticulum, mitochondria and peroxisomes in primary cultures of rat hepatocytes.
Correspondence
Kevin D. Mullen – 1 May 1987
Hepatology: Medical outline series. By Gary Gitnick. 384 pp. New York: Medical Examination Publishing Company, 1986, $37.50
James S. Morgan, Harold O. Conn – 1 May 1987
The jaundiced heart
Lawrence Deckelbaum – 1 May 1987 – Patients with obstructive jaundice are susceptible to postoperative shock and kidney failure. The cause of these potentially fatal complications has not been fully clarified. The present study was designed to assess the role of myocardial dysfunction in the hemodynamic disturbance of obstructive jaundice. We studied the effect of isolated cholemia on left ventricular performance in five conscious dogs before and 2 weeks after choledochocaval anastomosis by using measurements of systolic time intervals (STIs) and maximal dp/dt.