Characterization of ciprofibrate and clofibric acid as peroxisomal proliferators in primary cultures of rat hepatocytes

Dennis R. Feller, Yogendra Singh, Vilas R. Shirhatti, Thomas A. Kocarek, Cuo‐Tung Liu, Gopal Krishna – 1 May 1987 – We have determined the comparative activities of peroxisomal proliferators, ciprofibrate and clofibric acid on various hepatic parameters associated with endoplasmic reticulum, mitochondria and peroxisomes in primary cultures of rat hepatocytes.

Secretory component‐dependent binding of immunoglobuin A in the rat, monkey and human: A comparison of intestine and liver

Christopher K. Daniels, Douglas L. Schmucker – 1 May 1987 – The source and significance of immunoglobulin A in bile remains controversial. In the rat, and several other species, immunoglobulin A is transported through hep‐atocytes by a specific receptor, secretory component. In humans, immunohistochemical methods have indicated a distinct lack of receptors for immunoglobulin A on hepatocytes. Binding assays with 125I‐immunoglobulin A and membranes from hepatocytes and intestinal cells of the rat display secretory component‐dependent binding.

Establishment and characterization of a human combined hepatocholangiocarcinoma cell line and its heterologous transplantation in nude mice

Tastuo Murakami, Hirohisa Yano, Masafumi Maruiwa, Shigetaka Sugihara, Masamichi Kojiro – 1 May 1987 – A human cell line (KMCH‐1) derived from a surgical specimen of combined hepatocellular and cholangiocarcinoma has been established. The original tumor consisted of both hepatocellular carcinoma of the trabecular type and cholangiocellular carcinoma. This cell line has been maintained for 26 months through 75 passages. KMCH‐1 cells show characteristics of adenocarcinoma on light and electron microscopy. They proliferate in culture in a pave stone arrangement.

The in vitro production of Dane‐like particles: Is it live or memorex?

Francis A. Farraye, Jerome B. Zeldis – 1 May 1987 – Closed‐circular HBV DNA was introduced into cells of the established human hepatoma culture HepG2. The culture medium of one of 40 single‐cell clones contained HBV surface antigen (HBsAg), core‐related antigens (HBc/eAg), and HBV DNA sequences. HBV DNA and DNA polymerase activity were detected in particles resembling both nucleocapsids and complete virions (Dane particles). Intracellular integrated and extrachromosomal HBV DNA sequences were detected.

The evolution of changes in quantitative liver function tests in a rat model of biliary cirrhosis: Correlation with morphometric measurement of hepatocyte mass

John B. Gross, Juerg Reichen, Thomas B. Zeltner, Arthur Zimmermann – 1 May 1987 – The aim of this study was to determine the prognostic significance of functional changes in the liver during progression of cirrhosis. Liver function was quantitated weekly by the aminopyrine breath test (measuring microsomal function) and the galactose breath test (measuring cytosolic function) in rats made cirrhotic by bile duct ligation (n = 14) and in sham‐surgery controls (n = 9). Nine rats died spontaneously of cirrhosis.

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